cd14 selection macs purification Search Results


macs technology  (Miltenyi Biotec)
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macs cd14 positive selection kit  (STEMCELL Technologies Inc)
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cd14 antibody  (Miltenyi Biotec)
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FIG. 1. Cell surface expression of scavenger receptor CD36. Flow cytometric analysis of expression of CD36 in <t>CD14</t> circulating mono- cytes from nondiabetic control subjects (NDC), well-controlled dia- betic patients (WCD), and poorly controlled diabetic patients (PCD). After isolation by positive selection on the AutoMACS cell sorting system, CD14 monocytes were labeled with an anti–CD36-phyco- erythrin antibody. ‚, patients with type 2 diabetes; Œ, patients with type 1 diabetes; ‰, control subjects. Data are expressed as percentage of positive cells for CD36; n 13 (NDC), n 22 (WCD), n 27, (PCD); ***P < 0.0005.
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macs separation cd14 microbeads  (Miltenyi Biotec)
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FIG. 1. Cell surface expression of scavenger receptor CD36. Flow cytometric analysis of expression of CD36 in <t>CD14</t> circulating mono- cytes from nondiabetic control subjects (NDC), well-controlled dia- betic patients (WCD), and poorly controlled diabetic patients (PCD). After isolation by positive selection on the AutoMACS cell sorting system, CD14 monocytes were labeled with an anti–CD36-phyco- erythrin antibody. ‚, patients with type 2 diabetes; Œ, patients with type 1 diabetes; ‰, control subjects. Data are expressed as percentage of positive cells for CD36; n 13 (NDC), n 22 (WCD), n 27, (PCD); ***P < 0.0005.
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cd14 positive selection kit macs cd14 micro beads  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc cd14 positive selection kit macs cd14 micro beads
FIG. 1. Cell surface expression of scavenger receptor CD36. Flow cytometric analysis of expression of CD36 in <t>CD14</t> circulating mono- cytes from nondiabetic control subjects (NDC), well-controlled dia- betic patients (WCD), and poorly controlled diabetic patients (PCD). After isolation by positive selection on the AutoMACS cell sorting system, CD14 monocytes were labeled with an anti–CD36-phyco- erythrin antibody. ‚, patients with type 2 diabetes; Œ, patients with type 1 diabetes; ‰, control subjects. Data are expressed as percentage of positive cells for CD36; n 13 (NDC), n 22 (WCD), n 27, (PCD); ***P < 0.0005.
Cd14 Positive Selection Kit Macs Cd14 Micro Beads, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 1. Cell surface expression of scavenger receptor CD36. Flow cytometric analysis of expression of CD36 in CD14 circulating mono- cytes from nondiabetic control subjects (NDC), well-controlled dia- betic patients (WCD), and poorly controlled diabetic patients (PCD). After isolation by positive selection on the AutoMACS cell sorting system, CD14 monocytes were labeled with an anti–CD36-phyco- erythrin antibody. ‚, patients with type 2 diabetes; Œ, patients with type 1 diabetes; ‰, control subjects. Data are expressed as percentage of positive cells for CD36; n 13 (NDC), n 22 (WCD), n 27, (PCD); ***P < 0.0005.

Journal: Diabetes

Article Title: Activation of peripheral blood CD14+ monocytes occurs in diabetes.

doi: 10.2337/diabetes.54.9.2779

Figure Lengend Snippet: FIG. 1. Cell surface expression of scavenger receptor CD36. Flow cytometric analysis of expression of CD36 in CD14 circulating mono- cytes from nondiabetic control subjects (NDC), well-controlled dia- betic patients (WCD), and poorly controlled diabetic patients (PCD). After isolation by positive selection on the AutoMACS cell sorting system, CD14 monocytes were labeled with an anti–CD36-phyco- erythrin antibody. ‚, patients with type 2 diabetes; Œ, patients with type 1 diabetes; ‰, control subjects. Data are expressed as percentage of positive cells for CD36; n 13 (NDC), n 22 (WCD), n 27, (PCD); ***P < 0.0005.

Article Snippet: Human venous blood was collected in a lithium heparin tube and processed within 1–2 h. CD14 monocytes were isolated directly from total blood with a magnetic activated cell sorting system (AutoMACS; Miltenyi Biotech) using microbeads coated with CD14 antibody (MACS CD14 MicroBeads; Miltenyi Biotech).

Techniques: Expressing, Control, Isolation, Selection, FACS, Labeling

FIG. 2. CD36 and CD68 mRNA expression. Expression of CD36 mRNA (A) and expression of CD68 mRNA (B) in CD14 monocytes from control subjects (NDC), well-controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD). ‚, patients with type 2 diabetes; Œ, patients with type 1 diabetes; and ‰, control subjects. The mRNA level was quantified using real-time RT-PCR; n 12 (NDC), n 16 (WCD), n 16 (PCD). *P < 0.05; ***P < 0.005; ***P < 0.0005.

Journal: Diabetes

Article Title: Activation of peripheral blood CD14+ monocytes occurs in diabetes.

doi: 10.2337/diabetes.54.9.2779

Figure Lengend Snippet: FIG. 2. CD36 and CD68 mRNA expression. Expression of CD36 mRNA (A) and expression of CD68 mRNA (B) in CD14 monocytes from control subjects (NDC), well-controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD). ‚, patients with type 2 diabetes; Œ, patients with type 1 diabetes; and ‰, control subjects. The mRNA level was quantified using real-time RT-PCR; n 12 (NDC), n 16 (WCD), n 16 (PCD). *P < 0.05; ***P < 0.005; ***P < 0.0005.

Article Snippet: Human venous blood was collected in a lithium heparin tube and processed within 1–2 h. CD14 monocytes were isolated directly from total blood with a magnetic activated cell sorting system (AutoMACS; Miltenyi Biotech) using microbeads coated with CD14 antibody (MACS CD14 MicroBeads; Miltenyi Biotech).

Techniques: Expressing, Control, Quantitative RT-PCR

FIG. 3. DiI-OxLDL uptake by CD14 circulating monocytes. CD14 monocytes from control subjects (NDC), well-controlled diabetic pa- tients (WCD), and poorly controlled diabetic patients (PCD) were incubated in presence of DiI-OxLDL (50 g/ml) alone (A) or with an anti-CD36 monoclonal antibody (20 g/ml) (B) for 4 h at 4°C or 37°C. After washes and lysis of the cells, DiI-OxLDL uptake was assessed by spectrofluorimetry (excitation, 520 nm; emission, 580 nm). Data are expressed as means SE. For the DiI-OxLDL uptake experiments, n 7 (NDC), n 7 (WCD), n 9 (PCD); *P < 0.05, **P < 0.005. For the DiI-OxLDL uptake in presence of anti-CD36 monoclonal antibody, three samples per group were analyzed.

Journal: Diabetes

Article Title: Activation of peripheral blood CD14+ monocytes occurs in diabetes.

doi: 10.2337/diabetes.54.9.2779

Figure Lengend Snippet: FIG. 3. DiI-OxLDL uptake by CD14 circulating monocytes. CD14 monocytes from control subjects (NDC), well-controlled diabetic pa- tients (WCD), and poorly controlled diabetic patients (PCD) were incubated in presence of DiI-OxLDL (50 g/ml) alone (A) or with an anti-CD36 monoclonal antibody (20 g/ml) (B) for 4 h at 4°C or 37°C. After washes and lysis of the cells, DiI-OxLDL uptake was assessed by spectrofluorimetry (excitation, 520 nm; emission, 580 nm). Data are expressed as means SE. For the DiI-OxLDL uptake experiments, n 7 (NDC), n 7 (WCD), n 9 (PCD); *P < 0.05, **P < 0.005. For the DiI-OxLDL uptake in presence of anti-CD36 monoclonal antibody, three samples per group were analyzed.

Article Snippet: Human venous blood was collected in a lithium heparin tube and processed within 1–2 h. CD14 monocytes were isolated directly from total blood with a magnetic activated cell sorting system (AutoMACS; Miltenyi Biotech) using microbeads coated with CD14 antibody (MACS CD14 MicroBeads; Miltenyi Biotech).

Techniques: Control, Incubation, Lysis

FIG. 4. Expression of MCP-1 (A), ICAM-1 (B), and VCAM-1 (C) mRNA in CD14 monocytes from control subjects (NDC), well-controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD). Expression of gene mRNA level was determined by real-time RT-PCR; n 12 (NDC), n 16 (WCD), n 16 (PCD). Data are expressed as means SD,*P < 0.05. FIG. 5. Expression of PPAR (A), PPAR (B), and PPAR (C) mRNA in CD14 monocytes from control subjects (NDC), well-controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD). The mRNA level was quantified using real-time RT-PCR; 12 (NDC), n 16 (WCD), n 16 (PCD). Data are expressed as means SD,*P < 0.05.

Journal: Diabetes

Article Title: Activation of peripheral blood CD14+ monocytes occurs in diabetes.

doi: 10.2337/diabetes.54.9.2779

Figure Lengend Snippet: FIG. 4. Expression of MCP-1 (A), ICAM-1 (B), and VCAM-1 (C) mRNA in CD14 monocytes from control subjects (NDC), well-controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD). Expression of gene mRNA level was determined by real-time RT-PCR; n 12 (NDC), n 16 (WCD), n 16 (PCD). Data are expressed as means SD,*P < 0.05. FIG. 5. Expression of PPAR (A), PPAR (B), and PPAR (C) mRNA in CD14 monocytes from control subjects (NDC), well-controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD). The mRNA level was quantified using real-time RT-PCR; 12 (NDC), n 16 (WCD), n 16 (PCD). Data are expressed as means SD,*P < 0.05.

Article Snippet: Human venous blood was collected in a lithium heparin tube and processed within 1–2 h. CD14 monocytes were isolated directly from total blood with a magnetic activated cell sorting system (AutoMACS; Miltenyi Biotech) using microbeads coated with CD14 antibody (MACS CD14 MicroBeads; Miltenyi Biotech).

Techniques: Expressing, Control, Quantitative RT-PCR

FIG. 6. Circulating monocyte adhesion to HAoEC. After being labeled with CMFDA, CD14 monocytes from control subjects (NDC), well- controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD) were incubated in presence of HAoEC at 37°C for 45 min. After PBS washes, the numbers of attached monocytes per square millimeter were quantified on five randomly chosen fields, using an inverted microscope. Data are expressed as means SE. For monocyte adhesion experiments, n 10 (NDC), n 8 (WCD), n 6 (PCD), ***P < 0.0005.

Journal: Diabetes

Article Title: Activation of peripheral blood CD14+ monocytes occurs in diabetes.

doi: 10.2337/diabetes.54.9.2779

Figure Lengend Snippet: FIG. 6. Circulating monocyte adhesion to HAoEC. After being labeled with CMFDA, CD14 monocytes from control subjects (NDC), well- controlled diabetic patients (WCD), and poorly controlled diabetic patients (PCD) were incubated in presence of HAoEC at 37°C for 45 min. After PBS washes, the numbers of attached monocytes per square millimeter were quantified on five randomly chosen fields, using an inverted microscope. Data are expressed as means SE. For monocyte adhesion experiments, n 10 (NDC), n 8 (WCD), n 6 (PCD), ***P < 0.0005.

Article Snippet: Human venous blood was collected in a lithium heparin tube and processed within 1–2 h. CD14 monocytes were isolated directly from total blood with a magnetic activated cell sorting system (AutoMACS; Miltenyi Biotech) using microbeads coated with CD14 antibody (MACS CD14 MicroBeads; Miltenyi Biotech).

Techniques: Labeling, Control, Incubation, Inverted Microscopy